This project investigates the natural production of the thiopeptide antibiotic GE2270A by Planobispora rosea. Transcriptomic data reveal a shutdown of protein synthesis and respiratory chain at the end of exponential growth, that phosphate and iron limit growth under the fermentation conditions used, and a new biosynthetic gene cluster related to benarthin and dibenarthin production was discovered. A genome scale metabolic model, iFDC900, was also built.

Citation: Del Carratore F, Iorio M, Pérez-Bonilla M, Schmidt K, Pérez-Redondo R, Sosio M, Macdonald SJ, Gyulev IS, Tsigkinopoulou A, Thomas GH, Genilloud O, Rodríguez-García A, Donadio S, Breitling R, and Takano E. Multi-omics Study of Planobispora rosea, Producer of the Thiopeptide Antibiotic GE2270A. mSystems. 2021;6:e0034121. doi: 10.1128/mSystems.00341-21

Gene Details: EV45_RS13000